I have MCF-7 and MCF-10: what is the difference between them?

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What is the method in detail of reporter gene of luciferase assay ?

I will do the reporter gene by luciferase assay to study the promoter activity?i will use pgl3 basic vector and prl-tk,, the cell line MCF-7and MCF-7 , I will use lipofectamine 2000 and optimum...

04 May 2015 8,101 3 View

What is benefit added of BSA with digestion buffer ?

I have two restriction enzyme XhoI and HindIII , i used BSA only with digest vector not interested DNA , is it ok ?

04 May 2015 2,869 2 View

Does gene expression of any gene in breast cancer line reflect the gene expression in vivo in the breast ?

when we dealing the cell to convert to cell line , the property of the cell line like gene expression of cell or phenotype from the vivo tissue ? do we can depend the result expression of gene in...

02 March 2015 2,410 9 View

What is the normal level of concentration of TNF-alpha in rat colon tissue ?

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01 February 2015 8,389 0 View

Why did I not got any results with lkb1 primer in real time pcr?

I had mcf-10 cell and was grown in dmem/f12 media with fbs and AB and rna and dna was extracted from the cell ,, when I did RT-PCR with cDNA , I got results with GAPDH but not with my interested...

31 December 2014 9,071 3 View

Why the different size of band with the same gene in pcr ?

I have rna for MCF-10 and MCF-7 ....the concentration of rna of mcf-7 is 884 ng/microliter with 260/280 1.76 .. the concentration of rna of mcf-10 is 668 ng/microliter with 260/280 1.91 I convert...

31 December 2014 6,245 8 View

What are the reasons for low expression for the gene LKB1 in a mcf-10 cell line?

The MCF-10 cell line was grown with DMEM12, 10% serum, 5% antibiotic and HuMEC supplemented. When we studied gene expression of LKB1 by RT-pcr ROCHE the results is small in triplicate (CT 35 ,42...

31 December 2014 8,991 6 View

Can I use pipette tips that have water in them after autoclaving in RNA extraction?

When I autoclaved the tips that will be used in my DNA and RNA extractions, the tips had drops of water inside, although I covered the boxes of tips with foil.

11 December 2014 7,833 13 View

If I isolate DNA and RNA from cell monolayer culture by Trizol, do I need to centrifuge after adding Trizol and before adding choloroform?

My cells are MCF-7 and MCF-10. I want extract DNA and RNA from these cell using Trizol. Do I add Trizol directly into the flask after removing the media? What amount of Trizol is needed if I grow...

11 December 2014 2,579 5 View

What is appropriate media for MCF-7 cell and MCF-10 ?

I have mcf-7 and mcf-10 and I grow them in DMEM 12 media. Is this a suitable method?

10 November 2014 2,020 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

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04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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04 August 2024 7,282 2 View

Bashir Alsiddig Yousef Popular answer

MCF-7 is a breast cancer cell line with overexpression of estrogen receptor, while MCF 10A is a non-tumorigenic epithelial breast cell line.

Bashir Alsiddig Yousef

Saud Hasan

MCF 7 appropriate cell line for research purposes for the induction of breast cancer while MCF 10 used in research as control no growth of tumor