What is the method in detail of reporter gene of luciferase assay ?

I will do the reporter gene by luciferase assay to study the promoter activity?i will use pgl3 basic vector and prl-tk,, the cell line MCF-7and MCF-7 , I will use lipofectamine 2000 and optimum...

04 May 2015 8,038 3 View

What is benefit added of BSA with digestion buffer ?

I have two restriction enzyme XhoI and HindIII , i used BSA only with digest vector not interested DNA , is it ok ?

04 May 2015 2,818 2 View

Does gene expression of any gene in breast cancer line reflect the gene expression in vivo in the breast ?

when we dealing the cell to convert to cell line , the property of the cell line like gene expression of cell or phenotype from the vivo tissue ? do we can depend the result expression of gene in...

02 March 2015 2,360 9 View

What is the normal level of concentration of TNF-alpha in rat colon tissue ?

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01 February 2015 8,332 0 View

Why did I not got any results with lkb1 primer in real time pcr?

I had mcf-10 cell and was grown in dmem/f12 media with fbs and AB and rna and dna was extracted from the cell ,, when I did RT-PCR with cDNA , I got results with GAPDH but not with my interested...

31 December 2014 9,018 3 View

Why the different size of band with the same gene in pcr ?

31 December 2014 6,189 8 View

What are the reasons for low expression for the gene LKB1 in a mcf-10 cell line?

31 December 2014 8,928 6 View

Can I use pipette tips that have water in them after autoclaving in RNA extraction?

When I autoclaved the tips that will be used in my DNA and RNA extractions, the tips had drops of water inside, although I covered the boxes of tips with foil.

11 December 2014 7,777 13 View

If I isolate DNA and RNA from cell monolayer culture by Trizol, do I need to centrifuge after adding Trizol and before adding choloroform?

11 December 2014 2,515 5 View

What is appropriate media for MCF-7 cell and MCF-10 ?

10 November 2014 1,972 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View