What is the appropriate time to anneal e-beam evaporated gold on 2nm thickness Si02/Si wafers?

I want to get as much as the flatness of e-beam evaporated Au on Si02/Si wafers. So I want to anneal au deposited Si02/Si wafers. The thickness of Si02 is 2nm. Is there any appropriate time and...

01 December 2020 7,696 3 View

How to validate Protein dimerization?

I have expressed viral coat protein in BL21 cells. After purification of the protein, I am observing two bands, one of the size of the protein and one exact double size in SDS page. I think the...

01 June 2020 6,722 25 View

How do you study material (nano) interactions with Cancer Stem Cells ?

I would like to know what are the protocols, assays and theory to study a nanomaterial interaction with cancer stem cells (and not normal cancer cells).

15 December 2019 6,398 3 View

How do stable isotopes (not radioactive) form in stars and other bodies in the universe?

I was once told that stable isotopes of lighter elements such as H, N C , etc are found in stars, planets, etc. Can anyone suggest any literature which talks about the formation of these isotopes?

15 December 2019 6,535 3 View

Why vedic mathematics based algorithms are not seen in reputed journals of algorithms, Hardware Architectures?

Why vedic mathematics based algorithms for division, multiplication etc. are not seen in reputed journals of Algorithms, Hardware architectures.

01 May 2019 9,805 2 View

What is the significance of BET analysis of nanoparticles for drug delivery?

Do explain in term of BET based parameters such as BET Surface Area, Langmuir surface area, Adsorption-desorption volume of pores and pore diameter.

26 February 2019 9,079 5 View

How much time does it take for cellular uptake of nanoparticles in general?

Cancer cells uptake of NPs time period?

24 February 2019 6,710 5 View

How do we approach to cure Osteosarcoma using drug delivery systems?

There are lot of ways people are using drug delivery systems (DDS) to treat bone cancer, for instance: 1. Chemo drug loaded DDS 2. Gene DDS (mRNA) 3. Dual DDS 4. Drug- protein (growth factors,...

20 September 2018 1,591 3 View

Do any specific cation (not complexes) displays anticancer effects?

Could someone tell me any specific cation which have a role to kill the tumor cells, without magnetic or photo-thermal use?

06 September 2018 1,219 3 View

What are some in- vitro tests performed to evaluate anti- cancerous, anti- inflammatory and antimicrobial properties for a hydrophobic drug?

The drug is loaded in nano-particles and the nature of drug is highly hydrophobic.

20 June 2018 1,633 4 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

What are difference between laser diodes, simple light emitting diodes and power light emitting diodes when operated in the visible spectrum ?

I want to illuminate a scene (small space) using LED to capture photographs or videos of some tiny objects inside the space. But am not sure if I should use a Laser LED, or simple LED or power...

22 February 2021 4,240 4 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View