Im having trouble to obtain strong bands when using eric-pcr for e coli dna templates.
Are you following a published protocol? Depending on how much variation there is between different strains of E. coli, the primers may not be efficient or effective for your E. coli.
Do you have a set of control primers that you know always give a strong band? The other issue could the quality or quantity of your DNA template. Do some troubleshooting to see if you get good amplification from your DNA with another primer set.
You need to determine if the issue is with your DNA, your primers, the PCR reaction protocol or a combination of more than one factor.
Usually ERIC gives strong and weak bands. If your desired strong band were faint and rest of the bands were okay, recheck the PCR program and your bacterial isolates from which you extract the DNA . Can you share your gel image and the protocol?
hello Candelaria,
Try M13 primer which gives distict bands for clonal typing.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology