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Questions related from Nithin Sam .R
What is the significant fold increase for delta delta ct analysis for a prokaryotic gene. Is it above 2 fold? If so, have any one have got above two fold increase in any prokaryotic gene? Iam...
01 December 2016 6,179 3 View
Best buffer combination for lysis of fungal (mainly yeast) cell wall.
19 July 2016 9,871 3 View
will rna get amplified when using it as a template for PCR? -using dna specific primer -with gc% 55 -using high fedility polymerase.
28 June 2016 3,695 7 View
In case of bacterial total RNA, since all mRNA will not have poly A tail. I am using only random hexamer. Is it enough are should i add T tail oligomer also with the random hexamer. And what is...
27 June 2016 8,149 3 View
I need to know the approximate doubling time for wild type 1) E.coli 2)klebsiella pneumoniae 3)Acinetobacter baumannii 4)Pseudomonas aeruginosa Thank you.
14 April 2016 1,160 2 View
I want to know whether the RNA i have isolated from Klebsiella pneumoniae is in good condition. I would also like to know what the four bands are (there should be only 3 bands and 5s is not...
05 April 2016 9,324 5 View
I have done MLST for some isolates of Acinetobacter baumannii which are obtained from ICU scenario in a single month. In which five genes have same clonal number in all isolates while only two...
22 March 2016 1,259 2 View
