Nithin Sam .R

5 Questions 27 Answers 0 Followers

Questions related from Nithin Sam .R

Delta delta Ct analysis for prokaryotes?

What is the significant fold increase for delta delta ct analysis for a prokaryotic gene. Is it above 2 fold? If so, have any one have got above two fold increase in any prokaryotic gene? Iam...

01 December 2016 6,099 3 View

Will Total RNA get amplified using dna specific primer?

will rna get amplified when using it as a template for PCR? -using dna specific primer -with gc% 55 -using high fedility polymerase.

28 June 2016 3,629 7 View

Query in cDNA conversion in prokaryotes?

In case of bacterial total RNA, since all mRNA will not have poly A tail. I am using only random hexamer. Is it enough are should i add T tail oligomer also with the random hexamer. And what is...

27 June 2016 8,091 3 View

Can any one suggest the integrity of the RNA gel pic that i have attached?

I want to know whether the RNA i have isolated from Klebsiella pneumoniae is in good condition. I would also like to know what the four bands are (there should be only 3 bands and 5s is not...

05 April 2016 9,262 5 View

Regarding MLST (Multi Locus Sequence Typing) data in Acinetobacter baumannii using 7 house keeping genes?

I have done MLST for some isolates of Acinetobacter baumannii which are obtained from ICU scenario in a single month. In which five genes have same clonal number in all isolates while only two...

22 March 2016 1,169 2 View