Hello Kunal Bhosle

Yes, biomarkers are secreted by cancer cells, or they may be also generated by other non-cancer cells or tissue in response to cancer. Biomarkers can be DNA, RNA, protein or metabolomic profiles that are specific to the tumor. You may look at the DNA sequence, gene fusions, measure RNA or protein levels.

For K-562, I would suggest you focus on the exosomes released by K-562 cells into the conditioned media. These exosomes show angiogenic activity in both in vitro and in vivo matrigel assays. They have a role to play in the physiologic organization of endothelial cells. The exosomes may interact with their target cells in three ways namely, binding to cell surface receptors, fusion with the plasma membrane, or internalization. The exosome content could contribute to endothelial cell stimulation.

So, the ability of exosomes released by K-562 to interact with and stimulate endothelial cells could possibly suggest that exosomes could be a new target for chronic myeloid leukemia therapy.

You may want to refer to the article attached below. It will be helpful.

Article Exosomes released by K562 chronic myeloid leukemia cells pro...

Additionally, the phenotype of these cells include the immunological markers CD3 (−), CD13 (+), CD19 (−), CD34 (−), CD41 (+), CD42 (+), CD71 (+) and CD235a (+), and they carry the BCR/ABL fusion gene, which promotes cell growth, inhibits apoptosis and causes defects of DNA repair.

Please refer to the attached articles below for more information.

Article Downregulation of Bcr-Abl in K562 cells restores susceptibil...

Article Constitutive expression of platelet glycoproteins by the hum...

https://journals.viamedica.pl/acta_haematologica_polonica/article/view/83742

Best.