Hi,
So the whole blood samples are in RNA later solution and kept at -20 (500 ul blood+ 1000 ul RNA later). Please suggest protocol to discard the RNA later and process for RNA isolation. These RNA samples will be used for both sequencing as well as for cDNA synthesis
N.B: RNA later solution is from invirogen and the RNA isolation kit I will be using is from Qiagen.
Any help would be appreciated
Abhijeet Singh
Most of the RNA stabilizing solutions are compatible with most of the downstream steps and does not require any additional step to be discarded. As you will probably be using column based extraction there are several washing steps which will remove any traces.
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