Nilay Mitash

4 Questions 17 Answers 0 Followers

Questions related from Nilay Mitash

Can we transfect polarized epithelial cells grown on collagen coated inserts with siRNA by adding it directly into basolateral media?

I want to silence a gene in primary cells using siRNA approach. I have already seen effects of miRNA mimics in similar cell line but on different gene by adding them directly into media (7 days...

04 April 2019 4,802 4 View

Which kit is best for RIP (RISC immunoprecipitation) for exploring the interaction between miRNA-AGO2 and mRNA?

I want to see interaction between miRNA with some of its target mRNA through RIP. There are several kits available. I am just interested to know the best one. Can anyone help me?

07 July 2018 8,481 0 View

Can any one tell me about Universal Stem looped primer for miRNA cDNA synthesis? Is ThermoFisher Scientific making such primers?

I want to make cDNA of miRNAs so that I can quantify desired miRNA in future. I came to know that such primers are available. See link below: Current Protocols in Molecular Biology

06 June 2018 3,304 4 View

Can we quantify miRNA without enrichment and with random hexamer primer and sybr green chemistry?

Please suggest cost effective protocols for miRNA quantification.

01 January 1970 4,357 3 View