Isaiah Osei Duah Jr

2 Questions 3 Answers 0 Followers

Questions related from Isaiah Osei Duah Jr

LPS dose for RAW 264.7 NF-kB macrophage?

I am working on a project that requires stimulating RAW 264.7 NF-kB macrophage and readout my signal by luciferase assay. Although all concentrations tested (10, 50, 100ng/mL) after 24 hours...

17 June 2024 2,837 3 View

I did a Renilla luciferase assay and the empty wells do have a high absorbance readout. Also there is variation in my triplicates?

I use raw 264.7 NF-kB cell line and the empty wells has a high absorbance as close as the blank well.

21 May 2024 1,476 4 View