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As the title described, it is common that a target gene sequence contains motif that acts as the binding site of transcription factor rather then the upstream regulatory region. Does this affect...
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As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...
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My lab co-worker need a lot of T cell for his animal experiment, however one of the donor can't provide enough for downstream analyses, we thought to combine purified T cells from two donors in...
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I was trying to use Cytoscape to replace IPA to fit our labs convenience, also with no self-generated database or profiles I feel the need to start with the Biogateway app, however whenever I...
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Many articles describe the suspension cell growth is somewhat limited by concentration, that was observed in my experiment, doubling time of 1e5 cells/mL was longer than 5e5 cells/mL. The growth...
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Our department have Guava easyCyteTM8 flow cytometer which has a 488 and 640 laser and various shutter as described in link, https://www.aub.edu.lb/fm/medicalresearch/Pages/Guava.aspx , we...
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PAGE was done using Sigma-Aldrich TurboMix Bis-Tris Gel Casting Kit (TMKIT-10, TMKIT-60), we have cast total of 2 gels into this experiment, while in the end of PAGE, bands of protein ladder can...
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My experiment design requiring use of virus components from start to obtain a lentivirus that carry designated DNA to create a stable cell line, before infection we usually use a 50c.c. 100kDa...
23 August 2021 8,709 1 View
I'm now working on a large quantity of DNA cloning which have to repeatedly perform mini-prep and midi-prep. I was been told that some of the plasmids such as pUC57 or pLNCX can first be grow in 3...
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Destabilization domain(DD) is a strategy to combine a peptide sequence to a protein, which makes it unstable and vulnerable to proteasome for degradation. Upon binding of ligand with DD, the whole...
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