Mu-Shen Chang

9 Questions 42 Answers 0 Followers

Questions related from Mu-Shen Chang

What happen if a transcription factor bind directly to not only the regulatory region but also the target gene sequence?

As the title described, it is common that a target gene sequence contains motif that acts as the binding site of transcription factor rather then the upstream regulatory region. Does this affect...

14 May 2024 1,043 3 View

Antibody maleimide conjugation - TCEP method affected by tris concentration in purified buffer?

As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...

30 April 2024 3,194 2 View

Can I merge T cell from two different host?

My lab co-worker need a lot of T cell for his animal experiment, however one of the donor can't provide enough for downstream analyses, we thought to combine purified T cells from two donors in...

07 November 2023 7,150 0 View

Cytoscape loading node error?

I was trying to use Cytoscape to replace IPA to fit our labs convenience, also with no self-generated database or profiles I feel the need to start with the Biogateway app, however whenever I...

04 July 2023 9,359 0 View

Can Jurkat or Raji cell grow from single cell?

Many articles describe the suspension cell growth is somewhat limited by concentration, that was observed in my experiment, doubling time of 1e5 cells/mL was longer than 5e5 cells/mL. The growth...

10 June 2022 8,062 0 View

How to choose desired dye based on wavelength in flow cytometry?

Our department have Guava easyCyteTM8 flow cytometer which has a 488 and 640 laser and various shutter as described in link, https://www.aub.edu.lb/fm/medicalresearch/Pages/Guava.aspx , we...

06 June 2022 5,520 0 View

MOPS PAGE transfer result showed diffused protein ladder, what might be wrong?

PAGE was done using Sigma-Aldrich TurboMix Bis-Tris Gel Casting Kit (TMKIT-10, TMKIT-60), we have cast total of 2 gels into this experiment, while in the end of PAGE, bands of protein ladder can...

14 February 2022 4,439 0 View

How to condense virus sup. using centrifugal condenser?

My experiment design requiring use of virus components from start to obtain a lentivirus that carry designated DNA to create a stable cell line, before infection we usually use a 50c.c. 100kDa...

23 August 2021 8,654 1 View

Can E. coli LB mixture be transfer to a new fresh LB and shake for growing?

I'm now working on a large quantity of DNA cloning which have to repeatedly perform mini-prep and midi-prep. I was been told that some of the plasmids such as pUC57 or pLNCX can first be grow in 3...

13 July 2021 668 3 View