I am trying to isolate a protein by SDS PAGE but while running the gel I observe lot of minute bubble formation. will this effect the rate of migration and resolution of my gel?. i am applying a current of 50mA
the gel was extremely covered with bubbles than usual. thank you for the answers
Bubble formation is the indication of gel run, may be the voltage was higher than usual. Hope you have finished it properly.
Hi
Bubble formation means your system are working, but if you see it is more than normal, maybe you use a high voltage. Increase the voltage and try agaig.
Some amount of bubbling around the wire is normal indicating the electricity is being conducted. But if you are seeing excessive bubbling at low current that might indicate that there is a leak in the system, open up the cassette and reassemble the system, make sure the buffer is up to the mark between the two gels. Another thing you might want to check is the buffer itself, if you were reusing the buffer that could also cause excessive bubbling. Try making fresh electrophoresis buffer. If the buffer is off and the bubbles are too much it might affect the gel to some extent.
For anyone else who is having this issue and making their own gels, I would check your gel recipe . Having a too rigid gel due to errors in solution making can also result in way to many bubbles coming out of the electrode buffer.
- Badges
- Science method