I am looking for INS-1 cell line. Where I can purchase it?

More Mina Khalaji's questions See All

How can I adjust the cell number (cfu/ml) of Pseudomonas aeruginosa ATCC 27853 to OD600 nm absorbance and the McFarland standard?

Hi everyone Good time. I am working on Pseudomonas aeruginosa ATCC 27853 in our lab and I want to know whether there is any strong literature or previous data about the correlation between the...

13 May 2024 6,888 0 View

What do you advise me?

I want to read a book about human resources management. What do you advise me?

27 April 2024 2,645 3 View

How important is AI for marketing?

How can AI improve marketing for businesses?

19 March 2024 2,867 5 View

How to solve revelation problems of PVDF LF membranes on western blots ?

Hello, I have a problem when revealing my western blots ! I use biorad LF PVDF membranes with the Biorad transfer system (in 7 min) I block with the Biorad blocking solution then I incubate with...

18 January 2024 9,254 4 View

Why there are non-specific IL-5 secreting cells in the lungs of mice?

Hello all, I'm studying Th-1, Th-2 and Th-17 response in vaccinated and control mice groups. I perform T-cell cytokine Elispot (IFN-gamma, IL-5 and IL-17) on lung and spleen tissues. I've faced an...

31 July 2023 7,953 0 View

Is there any way to prevent murine splenocytes from clumping?

Hi, I am seeing cell clumps in single cell suspension of splenocytes. I use DMEM media+HEPES+Gentamycin. Then I resuspend the cells in AIM-V media. The cells get clumpy and they can even be seen...

31 July 2023 5,564 0 View

In hydrogel behavior what is the equation for degree of crosslinking and Flory-Huggins in the polymer-water interaction parameter?

What is the equations for degree of crosslinking and Flory-Huggins in the polymer-water interaction parameter?

26 April 2023 1,829 0 View

Can I ask somone to helpe me regarding interpretation of this TGA graph?

25 April 2023 4,460 5 View

How to analyze EVs NTA data in prism?

If I have 10 EVs samples data from NTA, I would like to run two groups t tests in prism for size, concentration and number of EVs, the thing is that, the raw data from NTA is too big for each...

20 April 2023 7,611 2 View

Can anyone help me to model of digital twin of a asset? OR How can build an account in amazon or azure in Iran?

I m a phd student and work in digital twin technology, but I have some limitations such as sign in cloud platform, work with digital model and ....

15 April 2023 997 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View