As we know, many blood group systems exist in a human being besides ABO and Rh blood group system. Is it feasible to identify the status of all blood group systems (33 systems comprise nearly 300 antigens) in a given blood sample? Especially, any high-throughput method since there is a need to screen large samples.
Hi Annadurai,
I came across some references for determining different minor red blood cell antigens through DNA analysis. Mainly we focus on the determination of common blood group system, however, there is a need to understand for other minor blood group systems too. Hope, your approach would be good for clinical practices in future. I am listing some of the common methods used for determining the minor blood group systems for your reference.
1. David J Anstee. Red cell genotyping and the future of pretransfusion testing. 2009 114: 248-256.
2. Hashmi et al. Determination of 24 minor red blood cell antigens for more than 2000 blood donors by high-throughput DNA analysis. Transfusion, 2007 Apr;47(4):736-47.
Good Luck!
Hi Annadurai,
You can identify all the blood group antigens, but it will be a real waste because some of them are clinically significant while the other are not.
You need to determine what a genotyping technique you are going to use depends on your facility.
Please see thess papers:
1- Blood group genotyping: from patient to high-throughput
donor screening. B. Veldhuisen, C. E. van der Schoot & M. de Haas
Vox Sanguinis (2009) 97, 198–206
2- The BloodGen project: toward mass-scale comprehensive
genotyping of blood donors in the European Union and beyond by Avent, D. et al
TRANSFUSION 2007;47:40S-46S.
I hope this will help.
Amr
Hi
I hope to make a review about blood ionization ( radiation interaction with the blood components )
Hi Asaad,
This is another subject. But actually radiation is used nowadays for pathogen inactivation for fresh-frozen plasma products.
As per literature microarray based blood grouping provides an opportunity to test multiple parameters simultaneously. As per Hashmi et al "By use of a microplate format, 96 DNA samples can be typed in approximately 4 hours, with only about 1 hour of “hands-on” time. The rate of throughput is further increased by “staggering” assay protocol steps and by parallel processing of multiple plates. The small feature size, corresponding to that of individual beads, and small overall footprint of the arrays not only ensure rapid probe-target capture and assay completion in 15 to 30 minutes, but also allow automated image acquisition by instant “snapshot” imaging with a simple fluorescence microscope equipped with a charged coupled device camera. Because the results are interpreted by software, an interface to the donor database will eliminate the need for manual entry of results."
Reference: http://onlinelibrary.wiley.com/doi/10.1111/j.1537-2995.2005.04362.x/full
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