Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

No title

26 December 2020 1,951 3 View

PLS-SEM generalizability issue?

I'm developing a readiness assessment model regarding contractors' preparedness for a specific activity, in order to do so, a survey study was carried out and the data analyzed with PLS-SEM to...

24 November 2020 399 1 View

Does anyone know where to order the dye Direct Yellow 96 (Solophenyl flavine) in Europe ?

I cannot find any supplier in Europe of this dye. Than you !

04 October 2020 9,305 2 View

What are the possible reasons for getting bands for both methylation and unmethylation primer set in MS PCR?

I am currently doing methylation specific PCR and I find that all my samples are showing bands for both methylation and unmethylation in both the control and tumor samples. I wonder is it because...

22 September 2020 6,002 4 View

What is the recommended tool for quality assessments of case reports?

I am performing a systematic review and the majority of published papers are case reports. In the same way we use the Newcastle-Ottawa scale (NOS) to evaluate the methodological design quality of...

13 September 2020 2,373 5 View

Is there any research on in-vitro meat about sporadic cancerous cells and their possible impact?

The high rate of proliferation required of stem cells could produce genetic instability which may result in sporadic cancerous cells. Is there any research on this?

10 September 2020 3,666 1 View

What are these bioclasts?

I'm having trouble identifying the round bioclasts indicated by white arrows. Could they be transverse sections of the elements indicated by black arrows? If so, could they be some kind of spicule?

22 July 2020 8,595 13 View

What is the proper solvent for crystallization of molebdenum diselenide in solution and room temperature other than DCM?

For crystallization we need a very good solvent to make a very clear solution, at least a good quantity of our solute is dissolved. However in our case we have to use an anhydrous solvent that...

17 June 2020 4,562 4 View

Is possible to run models with catergorical response variable and random factors?

Dear all, I would like to know if is possible to run a model with a categorical response variable plus some categorical predictors (and its interactions) and a random factor in R? If yes, any...

26 March 2020 1,221 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View