Alvaro del Real

2 Questions 4 Answers 0 Followers

Questions related from Alvaro del Real

Problem with RT-PCR. Enzyme alone with RNA, in RT, works. Why?

Hello everyone, I was just trying to do an RT-PCR with specific oligos to select the correct strand that I want to study. I cannot distinguish between both strands. So, I have tested different RT...

21 September 2018 1,726 3 View

How to transfect bone derived human mesenchymal stem cells with siRNA?

I am trying to transfect human mesenchymal stem cells with siRNAs. I've tried it with lipofectamine method but it didn't work. However, in other cell lines (like293T), it works. Is there any...

14 July 2017 1,076 2 View