Did anyone transfected a cell multiple times with same DNA?

Have anyone tried to transfect a cell multiple times with same DNA. Like a cell line which is already made stable with a particular DNA can be transfected again with the same DNA which is already...

09 October 2019 806 3 View

Did anyone have used Invitrogen Mini gel tank and western blotting?

Did anyone have used the invitrogen Minigel tanks for SDS PAGE and Western blotting. If so how was the performance and the reproducibility?

06 July 2019 6,938 8 View

What are the precautions to be taken during High fat diet feeding in mice? Precautions as in health maintenance and cleanliness and infections etc?

what precautions to be followed while feeding black 6 mice with high fat. what is the susceptible infections mice are prone to and what is basic care to be taken during high fat feeding?

06 July 2018 4,342 4 View

Did any one transfected RXR inducible GITR cotransfection in Jurkat cells using Lentivirus to get an inducible GITR expression?

Did any one transfected RXR inducible GITR cotransfection in Jurkat cells using Lentivirus to get an inducible GITR expression.

08 September 2016 3,040 0 View

Has anyone isolated/separated T cells from coculture?

i am coculturing t cells with other cell types and after co culture i want to isolate only t cells and eliminate other cells which this t cell is interacting so which method can i follow let it be...

01 February 2015 262 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View