Did any one transfected RXR inducible GITR cotransfection in Jurkat cells using Lentivirus to get an inducible GITR expression.
Have anyone tried to transfect a cell multiple times with same DNA. Like a cell line which is already made stable with a particular DNA can be transfected again with the same DNA which is already...
09 October 2019 806 3 View
Did anyone have used the invitrogen Minigel tanks for SDS PAGE and Western blotting. If so how was the performance and the reproducibility?
06 July 2019 6,938 8 View
what precautions to be followed while feeding black 6 mice with high fat. what is the susceptible infections mice are prone to and what is basic care to be taken during high fat feeding?
06 July 2018 4,342 4 View
07 August 2016 9,268 3 View
i am coculturing t cells with other cell types and after co culture i want to isolate only t cells and eliminate other cells which this t cell is interacting so which method can i follow let it be...
01 February 2015 262 7 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
02 March 2021 3,060 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View