Have anyone tried to transfect a cell multiple times with same DNA. Like a cell line which is already made stable with a particular DNA can be transfected again with the same DNA which is already integrated into the genome?
Yulia Panina
Yes, it can be transfected, in principle. I'm not sure what you are trying to achieve though, and what kind of transfection you are talking about. Retroviral? Plasmid? CRISPR? The answers about what will happen will differ depending on it.
0 votes
0 thanks
Sandra Lagauzère
not sure to understand what is your objective really, but YES you can transfect your stable cell line with anything you want.
0 votes
0 thanks
Andreas Eisenreich
I agree, yes you can transfect your stable cell line with the same and/or any other nucleotide sequence
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line
More Beera Shankar Anand's questions See All
Similar questions and discussions