23 Questions 62 Answers 0 Followers
Questions related from Hyde Chen
1.I want to isolate brown adipose from mice. I am not sure whether the tissue is right？（show in the attachment.) 2. I want to digest brown adipose to collected single cell sample. After 1 hour...
04 April 2018 2,748 0 View
I want to find the list of mice receptor ligand binding.
03 March 2018 6,079 3 View
1. western and RT PCR show the protein is high expression. 2.FCM show opposite result. 3.The antibody is different. ^^^^^The protein have been modified after translate?
12 December 2016 2,281 3 View
I want to do Ex Vivo Functional Analysis of human Dendritic Cells. What i found was here: 1,Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay (Principal...
10 October 2016 311 1 View
Read 3 answers by scientists with 1 recommendation from their colleagues to the question asked by Hyde Chen on Sep 21, 2016
09 September 2016 9,723 3 View
I got some NSG-like mice from company(in China). Those mice can survival in lab gangway（not clear） for almost 2 weeks. Does it normal? I want to check their blood through FCM. And I want to use...
09 September 2016 7,200 3 View
I want to check several genes in one single cell, What kit can i use?
09 September 2016 4,147 0 View
I designed many primers and they will work as a mixture. I am not sure whether those primers bind with each other (instead of template). I try to find some soft to check the quality of those...
07 July 2016 8,548 4 View
HSC are Cryopreserved. After thawing, HSC are used directly or culture on SFEM for a while before bone marrow transplantation（human HSC to mice）？ Does Cryopreserving affect the activity of HSC.
07 July 2016 1,207 1 View
I use the 4034 from stemcell. Seed cell are cd34+ from hES.
07 July 2016 4,435 4 View
A friend has a patient with a new mutation (leukemia). He want to keep this mutation to further study. So he collected monocyte from patient and want to make a immortal cell line. He do not know...
06 June 2016 1,977 5 View
HSC transplantation via mice tibia is easy for HSC homing. So i want to learn this method. But I did not find video or other protocol. I am not sure my practice is right. Some one know how to do...
05 May 2016 4,325 1 View
After Dox induce,some cells have red fluorescence, some cells do not? why? (RTTA-puro and ef1a-gfp-TRE-mecherry are in two LV)
12 December 2015 1,175 1 View
I cultured human CD34+ cells in 96 # 7days for randomly differentiated. Some cells(I am not sure…) are “bold". What are those big cells? I marked them in the pdf file.
09 September 2015 2,313 1 View
Most papers I read use NSG mice to test the quality of human HSC or progenitor cells derived from other cells (hESCS or fibroblast). Those mice have immune deficiency which confirm the survival of...
04 April 2015 9,615 2 View
I want to do hESC differentiation with some TFs, which work at different stages. The ef1a promoter drive genes without control. Dox-TRE system can only control one stage? How to control different...
03 March 2015 4,094 6 View
MLR and ELISPOT----- mix the T cells with the stimulator（cells），which one is more sensitive to the stimulator?
01 January 2015 6,109 5 View
"Three classes of stem cells exist, distinguished by their ratio of lymphoid to myeloid progeny (L/M) in blood. Myeloid-biased (My-bi) HSC have low L/M ratio (between 0 and 3), whereas...
01 January 2015 1,570 0 View
I am the first student of my boss in the University.He just focus on his business of his company (all the students in our lab is listless). It is hard to deal with him. It is a hard and common...
12 December 2014 7,990 0 View
To check the lymphocyte proliferation, I separated PBMC from human blood and incubated them with CFSE. Then I check the FITC immediately. The FCM show two clusters in the FITC, why the cell get...
12 December 2014 8,518 6 View
I want to stop the proliferation of human fibroblast. I try it with x-ray 500rad as a paper said. The cell proliferation was not affected. the irradiation from paper is from 500-6000. I do not...
11 November 2014 6,378 7 View
I do the mice BMT from August. The control mice died after 2 week. And the BMT mice were survived after one month. Then I kill the mice to check the GFP(I use the C57 GFP transplant to C57...
11 November 2014 8,762 9 View
human HSC can been transplanted in mice(NSG,NOG,NOD/SCID) through caudal vein or right femur (RF). Chimeric rate in PBMC and BM, which is higher? Can different transplant ways cause difference of...
01 January 1970 2,368 11 View
