How to select a appropriate linker for construction of a DNA nano-structure?

More Mokhtar Nosrati's questions See All

MANOVA for IV and DV catergorical data?

Hi everyone, I would like to know if there is any analysis by MANOVA for the categorical data of IV and DV. How is the step to running?

20 May 2024 4,066 1 View

For ns pulsed high voltage power supply (for atmospheric and vaccum plasma), which is better to order: positive or negative pulse? and why??

Many thanks in advance.

15 January 2024 6,145 4 View

What practical methods are used for abdominal ultrasound in ruminants?

We're talking about repeatable teaching methods and clinical practice methods.

07 June 2023 6,404 1 View

Please remove my article Methylene Blue Removal Using Black Cumin Seeds Waste: Experimental and Theoretical Investigations from the SSRN Electronic ?

Hello, I am hereby informing you that the article entitled: Methylene Blue Removal Using Black Cumin Seeds Waste: Experimental and Theoretical Investigations, has been hacked by other people . I...

30 March 2023 1,053 2 View

Difference hardness and elastic modulus?

hallo every one, I know that the hardness of a substance means how strongly it can resist against penetration of a harder substance and the E means after the release of a stress the material will...

21 February 2023 5,182 3 View

Adhesion Theory that describe the adhesion of the photo resist on wafer?

Hello every body, can some one please tell me which adhesion theory could describe the adhesion of photo resist on Wafer?? and with HMDS? thank you very much!!

10 January 2023 722 0 View

Effect of the implantation on hardness from the photo resist?

Hallo, i did a nanoindentation on an implanted CAR and i found that when the CAR has a thicker layer, then it is less hard. Does anyone know why?? thank you very much!!! best regards chiko

28 August 2022 4,657 0 View

What can affect the hardness of CAR (layer thickness)?

Hello, i did a nanoidentation experiment : 1 CAR with 3 different layer thicknesses. My results show that the CAR is harder when it has thicker layer.. I can't find the reason in the...

11 August 2022 2,888 0 View

What can affect the adhesion of CAR?

Hello, i am making researches about what can affect (or what could improve) the adhesion of CAR if any one have articles for this topic, please let me know. Thank you very much!! best regards chiko

03 August 2022 7,146 3 View

There is a database/resource to search whether the plant species are polyploid or diploid?

There is a database/resource to search whether the plant species are polyploid or diploid. I have many plant species and want to know which one is polyploidy/diploid.

23 June 2022 4,890 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I need JCPDS file of LSFCO nanomaterial. Can anyone provide me?

Require for Rietveld refinement in XRD.

08 August 2024 3,081 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are the apoptotic cells is positive for γH2AX ?

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28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Mohammed H. Musleh Popular answer

Hi dear Mokhtar

You can read the source in below that can generate the construction idea...

http://www.springer.com/la/book/9781493964529

Peter Allen

Depending on the structure you want to build, you can check out CADNANO

http://cadnano.org/

but it seems to have a steep learning curve. Here are early example structures:

http://www.nature.com/nature/journal/v459/n7245/abs/nature08016.html

Mohammed H. Musleh