How do we decide BSA percentage in our buffer solution?

Hi all, I am trying to test my polymer stability in biological medium. I know how to make 1% /5% etc of BSA in buffer but I am not sure how do we actually decide this percentage volume. Is there...

05 January 2021 649 2 View

Prevent contamination of solution in oven?

Hi all, I am trying to test stability of my electrode immersed in solution. I intent to keep them in oven for 3 weeks as to represent accelerate ageing. However, my worry is that the solution...

28 October 2020 8,351 2 View

Can graphene layers be coated by CVD on hydroxyapatite powders?

Is the hydroxyapatite decomposed during the CVD process?

29 February 2020 5,443 3 View

What is characteristic peak(s) in FTIR spectrum of crude plant extract?

Hello, I synthesized chitosan nanoparticles loaded with a plant extract. For confirming presence of the extract in the nanoparticles I did a FTIR analysis. Now my question is: What is...

24 January 2020 4,402 2 View

What do you suggest to me like books, articles to start working with isogeometric analysis?

I am looking to start working with the isogeometric method to analyze mechanical behavior such as beam, plate, shell and other structures of complex shapes. can you help me by giving me simple...

28 December 2019 2,729 5 View

How to ensure that a chemical synthesized peptide has correct sequence?

Dear all, I have ordered a peptide (with 32 aa) for chemical synthesis, but I dont know after receive a peptide how to ensure from its sequence. Which method(s) are available for my purpose?...

09 December 2019 3,784 3 View

How to measure fintech in banking sector?

Hi, I am currently pursuing my Phd study whereby my topic related to fintech and the effect towards green banking, and I am now in the process of understanding the LR about Fintech. Since Fintech...

08 December 2019 1,391 5 View

How to measure Moderator variable with SPSS?

Dear researchers, I want to learn how to measure moderator variable with SPSS. If you have any resources or videos i will appreciate to share it with me.

23 June 2019 9,504 3 View

Can anyone send to me a free download plagiarism checker program?

I found many plagiarism programs that allow only 150 words for checking. I search for a free download plagiarism checker program (not trial). Thanks

20 March 2019 5,708 6 View

Simple examples ,books and papers about The generalized differential quadrature method (GDQ) ?

I want to start working on the generalized differential quadrature (GDQ) method to solve the problem of the mechanical behavior of the beams, the plates and the shells. Dear colleagues , I...

13 November 2018 7,650 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View