How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Generate a structure file through PGDSpider?

Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...

29 December 2020 5,169 1 View

How big can a enhancer deletion be ? how can we obtain homologous deletion clone ?

Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...

13 December 2020 942 3 View

What is the viability and time taken for turmeric seeds?

Turmeric is a polyploid. Mostly sterile. However, it sets seed occassionally. I have collected seed and kept in poretrays filled with cocopeat. I have not observed any germination even after 15...

28 August 2020 8,342 4 View

If we cross Diploid plant (Female Parent) x Tetraploid plant. How much chances of getting viable seed?

In case of guava crossing of Diploid (Psidium guajava, 2n = 22) with P. Molle (2n=44). Is there any chance to get viable seed set?

16 June 2020 9,317 3 View

What do each of the peaks in a FCM graph represent for polyploid individuals?

I have been looking for an explanation or a graph that explains what each peak represents for a polyploid individual. I understand they ar the dna content of nuclei, but, 1) do they represent...

07 November 2019 509 3 View

Do polyploid plants have multiple cells of various ploidy, as in 2n, 3n, 4n, 6n, or is the leaf/plant tissue strictly 3n? Any good sources to review?

In most Flow Cytometry (FCM) graphs in journal articles of polyploid tissue, they show evidence of polyploidy as multiple peaked histograms. Does leaf tissue of a polyploid have 2n, 3n, 4n, and...

08 September 2019 6,256 1 View

Do polyploid plants have multiple cells of various ploidy, as in 2n, 3n, 4n, 6n, or is the leaf/plant tissue strictly 3n? Any good sources to review?

In most Flow Cytometry (FCM) graphs in journal articles of polyploid tissue, they show evidence of polyploidy as multiple peaked histograms. Does leaf tissue of a polyploid have 2n, 3n, 4n, and...

08 September 2019 9,527 1 View

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

26 June 2019 9,059 2 View

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

05 June 2019 1,890 2 View

Is there a yeast fluorescent reporter system that glows 1 color for a type, and another for alpha-type?

I'm trying to find a yeast fluorescent reporter system that will glow one color when the yeast is a-type, and a different color when the yeast in alpha-type. Icing on the cake would be a third...

01 February 2019 1,602 1 View