Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Is there a study of a long-term usage of muscimol and its impact upon human physical and mental health?

In my psychotherapeutic practice I have a client who decided to use really small doses of muscimol regularly. He gains it from dried fruit bodies of Amanita muscaria. Although he is quite enjoying...

14 December 2020 6,020 2 View

Covid 19 deaths- why the huge disparity between Asian countries which cannot be convincingly explained by public health measures?

Sri Lanka, Vietnam, Thailand, Cambodia and Laos for example are doing extremely well, but Indonesia, Philippines, Malaysia and India not so. It doesn't seem likely that this can be wholly due to...

27 October 2020 1,795 7 View

I see a lot of research right now that is inspired by COVID-19, even outside the medical field. Has your research focus changed due to the pandemic?

My question says it all. I am wondering if research agendas across many fields have changed due to the COVID-19 circumstances, and why.

06 July 2020 3,505 6 View

Can you help with an urgent survey about teaching during COVID-19?

Update: Data collection is closed and our manuscript has been published. Thank you to everyone who participated. You can find it at: Teacher Experiences in Converting Classes to Distance...

06 May 2020 1,701 46 View

Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

18 February 2020 2,386 1 View

Background noise in q-exactive orbitrap ms ?

Dear Colleges MS users. This is my very first thread on research gate. I have a new instrument - q-exactive orbitrap and i have experienced a high background ms noise in low m/z range. After...

29 January 2020 6,927 2 View

I'm looking for a laboratory-scale hydrogen separation membrane from the air?

dear all, I'm looking for a small-scale membrane for hydrogen separation from the air (or nitrogen). I need some information about available manufacturers. There is a lot of information about the...

15 December 2019 8,333 2 View

Is there anyone who could share the protocol for NLRP3 staining in the mouse paraffin embedded tissue?

Hello, I am looking for someone who performed already some IHC/IF staining in paraffin sections in mouse tissue for NLRP3. I used ab214185 and NBP2-12446 but no success so far. Thank you in advance

30 October 2019 4,354 1 View

Abaqus python, extract displacements of surface nodes, how?

Hi, I need to extract, from existing odb, displacements of nodes whhich belong to defined element surface. I use python. Till now I'm able to list the nodes labels and coordinates: N_set =...

27 March 2019 955 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View