PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

To become an Ethologist, what classes should I take freshman year of college to set me on that path?

I am currently a freshman in college aiming to become an Ethologist. I am wondering what classes I should take freshman year to set me on that path. My ultimate goal is to study big cats (lions,...

15 November 2020 5,212 15 View

Are there any research papers on the anatomy of big cat hybrids, such as ligers and tigons?

This is certainly going to be a strange question. Brace for impact. I performed very superficial searches on a couple of article databases (including researchgate.net). All I have found was one...

22 March 2020 1,142 3 View

Can the extinct ice age cave lions be cloned?

there was a news, 2 ice age cave cubs were found in the last august.so,can the DNA be extracted and cloned to regenerate the extinct cave lions?

07 August 2018 7,148 0 View

Can anyone recommend good audio recording equipment for large carnivore (specifically spotted hyena and lion) calls?

The recorded calls will later be used for playback experiments. I currently have an excellent FOXPRO speaker system for playback experiments, but am on the market for a high-quality microphone...

01 February 2018 685 6 View

May Inuits be alike Maasai warriors?

Maasai - the traditional lion hunters are becoming lion guardians, nowadays, when number of lions drop to fewer than 30 thousand. https://www.youtube.com/watch?v=Au5yE0Z2HUo The number of...

31 December 2017 8,752 2 View

If a lion was about to attack a zebra and a human being could save the zebra, are there circumstances where such an intervention would be justified?

If so, what would these circumstances be like? If not, why not?

11 December 2017 4,717 19 View

Why battery model performance increases with temperature ?

I validated Lion 26650 battery data against 2RC equivalent circuit model. The RMSE seems to improve with high temeparature ie good results for 50 deg, then 25 deg and 0 deg had bad results. What...

07 August 2017 4,400 6 View

What are the lastest mean-field price formation model?

I am interesting in some partial differential equation models in social-economic context. I know the model introduced by Lasry and Lions in 2007. Do you know anything similar?

07 August 2016 7,291 0 View

Do you see Mountain lion footprints as part of your work?

03 April 2016 3,752 1 View

Anybody has the research paper on Make in India. pl. share your theme

02 March 2016 4,833 0 View