Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Is there a study of a long-term usage of muscimol and its impact upon human physical and mental health?

In my psychotherapeutic practice I have a client who decided to use really small doses of muscimol regularly. He gains it from dried fruit bodies of Amanita muscaria. Although he is quite enjoying...

14 December 2020 6,020 2 View

Covid 19 deaths- why the huge disparity between Asian countries which cannot be convincingly explained by public health measures?

Sri Lanka, Vietnam, Thailand, Cambodia and Laos for example are doing extremely well, but Indonesia, Philippines, Malaysia and India not so. It doesn't seem likely that this can be wholly due to...

27 October 2020 1,795 7 View

I see a lot of research right now that is inspired by COVID-19, even outside the medical field. Has your research focus changed due to the pandemic?

My question says it all. I am wondering if research agendas across many fields have changed due to the COVID-19 circumstances, and why.

06 July 2020 3,505 6 View

Can you help with an urgent survey about teaching during COVID-19?

Update: Data collection is closed and our manuscript has been published. Thank you to everyone who participated. You can find it at: Teacher Experiences in Converting Classes to Distance...

06 May 2020 1,701 46 View

Precipitates in BL buffer (Epitect DNA bisulfite Kit)?

18 February 2020 2,386 1 View

Background noise in q-exactive orbitrap ms ?

Dear Colleges MS users. This is my very first thread on research gate. I have a new instrument - q-exactive orbitrap and i have experienced a high background ms noise in low m/z range. After...

29 January 2020 6,927 2 View

I'm looking for a laboratory-scale hydrogen separation membrane from the air?

dear all, I'm looking for a small-scale membrane for hydrogen separation from the air (or nitrogen). I need some information about available manufacturers. There is a lot of information about the...

15 December 2019 8,333 2 View

Is there anyone who could share the protocol for NLRP3 staining in the mouse paraffin embedded tissue?

Hello, I am looking for someone who performed already some IHC/IF staining in paraffin sections in mouse tissue for NLRP3. I used ab214185 and NBP2-12446 but no success so far. Thank you in advance

30 October 2019 4,354 1 View

Abaqus python, extract displacements of surface nodes, how?

Hi, I need to extract, from existing odb, displacements of nodes whhich belong to defined element surface. I use python. Till now I'm able to list the nodes labels and coordinates: N_set =...

27 March 2019 955 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View