How to preserve leukocytes for epigentic analysis?

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Max. shear stress in line contact with tangential load?

I'm analyzing the sub-surface stresses in a line contact in combination with a tangential load (sliding cylinder). I can calculate Sxx and Szz (Z is the normal to the contact surface). And Syy is...

23 November 2020 894 0 View

Can I reuse the reagent for protein transfection?

I have a question about protein transfection. I used a reagent to transfect a protein. Can I use the same reagent for a different protein that I want to transfect in the same cell line?

31 May 2020 2,750 3 View

What is the optimal primer concentration in qPCR?

Hello, In qPCR, is there an optimal concentration for primers with a total volume of 20ul per reaction? Thanks for your answer in advance!

24 May 2020 2,092 9 View

Is there a certain duration for gene knockdown using shRNA or is the knockdown permanent?

Can somebody help me with the following question: Is there a certain duration for gene knockdown using shRNA or is the knockdown permanent? Thanks and stay save!

05 April 2020 7,069 2 View

Which temperature for expressing thermophillic genes in E.Coli?

Good morning, Right now, I’m trying to express thermophilic genes in E.coli. On the internet I have read that I need to incubate the cells at 42 degrees for a short time period after IPTG...

08 March 2020 7,747 1 View

Does de presence of EDTA affect cDNA synthesis?

I want to synthesize cDNA for qPCR using the iScript cDNA synthesis kit. I suspended the RNA in TE buffer with 1mM EDTA. Does the EDTA have an influence on the synthesis of the cDNA. Note: the...

23 February 2020 7,798 3 View

How do stationary and dynamically induced resistance in fixed seat rowing compare with respect to magnitude?

Fixed seat rowing introduces pitching and heaving motions, especially at the start of the recovery, as well as a surge. How does the magnitude of the thus added resistance compare to the linear...

10 April 2016 5,952 1 View

What is the lowest shrinkage attained with polypropylene upon thermal processing?

What was the main variable? Backbone or blend composition, or other? I am looking for a grade or blend or compound of polypropylene with the lowest possible shrinkage upon cooling after thermal...

28 October 2015 4,874 4 View

If I want an accelerometer chip inside the same package as my ASIC, what trouble can I expect with an MCM?

Inertial MEMS give a small capacitive signal and are sensitive to vibrations. In a multi-chip module (MCM) wire bonding is applied to connect with a sense and control circuit (ASIC). What are the...

03 June 2014 8,892 4 View

Can we assess 'self-management exposure' in routine chronic care consultations?

In the Netherlands self-management for chronic disease patients has been adopted in routine care in both primary and secondary care. Evidence suggests high variance in succes of self-managment...

23 December 2013 281 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Method of collection of samples of microorganisms from environmets to labortary?

can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...

17 July 2024 3,687 11 View

Do you agree that at some point in our lives each of us will be a person with disabilities?

There is several approaches towards the analysis of legal status of people with disabilities (e.g. medical, social or human rights approach). One way to include people with disabilities in society...

17 July 2024 3,798 13 View

Can we preserve the protoplasts?

Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?

16 July 2024 9,576 1 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

Lenin Yong

Jaap,

If you are going to perform DNA methylation profiling, i would advice you to promptly extract DNA in order to avoid exposition to any extra stress conditions that could potentially induce unwanted methylation changes in your samples. DNA can be stored safely for long times in the freezer.

Liying Yan

If you do not want to extracted the DNA, the cell pellets can be stored at -20 freezer in genomic lysis buffer (ZymoResearch, Cat#D5021-9).

Stephen Fôn Hughes

I agree with Lenin Yong 's comment above, i.e. Quicker the better for extracting DNA

Stephen