Can I reuse the reagent for protein transfection?

I have a question about protein transfection. I used a reagent to transfect a protein. Can I use the same reagent for a different protein that I want to transfect in the same cell line?

31 May 2020 2,634 3 View

What is the optimal primer concentration in qPCR?

Hello, In qPCR, is there an optimal concentration for primers with a total volume of 20ul per reaction? Thanks for your answer in advance!

24 May 2020 1,801 9 View

Does de presence of EDTA affect cDNA synthesis?

I want to synthesize cDNA for qPCR using the iScript cDNA synthesis kit. I suspended the RNA in TE buffer with 1mM EDTA. Does the EDTA have an influence on the synthesis of the cDNA. Note: the...

23 February 2020 7,647 3 View

More than one public file for one publication is NOT user-friendly!

People have asked me why there is only an erratum for this publication: Identification for development it is not. 'Inclusive and tru... . Indeed, when going to "Public files (2)" only the later...

01 January 1970 4,883 2 View

DNA extraction of insects

I want to maximize the yield of DNA extraction from insects. I am already using different DNA isolation kits and I know that these are not specifically designed for insect samples. Does anyone...

01 January 1970 847 3 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

Can I publish my published dataset (data-report article) later as an original article?

I want to quickly publish a data-report .Because there is a lot of data and it will take time to create the original article. But my main goal is to use that data-set in the original article. Is...

02 February 2021 9,779 7 View

Does anybody has the biodot zx1010 manual?

25 January 2021 6,257 1 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

The > 91 Years long collective IQ Blunder ?

Never before was there a bigger blemish. Namely: Not to collectively underastand Zwicky's discovery of Cryodynamics, as his sister to deterministic Thermodynamics came to be called later. Dec....

30 December 2020 8,300 5 View

Is explanatory sequential design the right design for my research?

I am interested in finding the effects of technology on the learning achievement of students. The data collection will include pre and post test scores of the experiment group and later...

26 December 2020 3,757 4 View