Does de presence of EDTA affect cDNA synthesis?

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Anton Slyvka

Hi,

it depends on final EDTA concentration. Just take into account that EDTA binds Mg2+ with 1:1 stoichiometry and (if needed) resuply the reaction with additional Mg2+.

Good luck!

Martin Holub

Anton Slyvka, what is the reasoning behind the 1:1 stochiometry? Thanks!

Dear Martin,

it was empirically determined: Article Stoichiometry of Titration of Metal Ions with Disodium Salt ...

https://www.lookchem.com/Calcium-edta-complex/