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Questions related from Jaap Kleijkamp
I have a question about protein transfection. I used a reagent to transfect a protein. Can I use the same reagent for a different protein that I want to transfect in the same cell line?
01 June 2020 2,717 3 View
Hello, In qPCR, is there an optimal concentration for primers with a total volume of 20ul per reaction? Thanks for your answer in advance!
25 May 2020 1,979 9 View
Can somebody help me with the following question: Is there a certain duration for gene knockdown using shRNA or is the knockdown permanent? Thanks and stay save!
06 April 2020 7,055 2 View
Good morning, Right now, I’m trying to express thermophilic genes in E.coli. On the internet I have read that I need to incubate the cells at 42 degrees for a short time period after IPTG...
09 March 2020 7,727 1 View
I want to perform DNA methylation profiling in leukocytes for an epigenetic analysis. However, I want to preserve the leukocytes for DNA methylation profiling for later. What is the best way to...
02 March 2020 7,665 3 View
I want to synthesize cDNA for qPCR using the iScript cDNA synthesis kit. I suspended the RNA in TE buffer with 1mM EDTA. Does the EDTA have an influence on the synthesis of the cDNA. Note: the...
24 February 2020 7,768 3 View
I want to maximize the yield of DNA extraction from insects. I am already using different DNA isolation kits and I know that these are not specifically designed for insect samples. Does anyone...
01 January 1970 913 3 View
