I have a question about protein transfection. I used a reagent to transfect a protein. Can I use the same reagent for a different protein that I want to transfect in the same cell line?
I think you can, but take care of all conditions like storage, temp, and contamination.
Hi Jaap Kleijkamp ,
in our lab, we work with one transfection reagent for multiple proteins transfection into same cell line (in co-transfection too) without any problems. But it deffinitely depend on which transfection reagent you use. We work with FuGENE reagent (from Promega) and it 100% works with mamalian cells and insect cells too (maybe not that high passages...).
Good luck,
Matej
The success is depending on your tranfection reagent... the co-transfection (multiple plasmid DNA) works very fine if you are not using too much of the DNA amount (read company instructions). The tranfection one by one is also working- but depending on your cell line and plasmid DNA you should wait 24 - 48 hours between your next transfection (otherwise too much cells will die).
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