Which temperature for expressing thermophillic genes in E.Coli?

09 March 2020 1 8K Report

Good morning,

Right now, I’m trying to express thermophilic genes in E.coli. On the internet I have read that I need to incubate the cells at 42 degrees for a short time period after IPTG induction. Is this true? Or what temperature should I use? Moreover, is this going to affect the protein?

Muhammad Ali

I suggest you to follow : https://www.researchgate.net/post/Why_cant_thermostable_protein_expressed_in_E_coli_tolerate_heat-treatment

Article Assembly and Multiple Gene Expression of Thermophilic Enzyme...

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