How to prepare Tris-acetate?

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John Hardy Lockhart

Yes. You should prepare a 40 mM Tris-base solution and adjust to pH 7.8 using glacial acetic acid.

Gabriel Salazar Robles

Ok, thank you!!!

Satwik Majumder

Hi Gabriel Salazar Robles

You can refer to :

https://www.sigmaaldrich.com/CA/en/technical-documents/protocol/protein-biology/gel-electrophoresis/tae-and-tbe-running-buffers-recipe