I need to know how to prepare primary chicken embryo fibroblast and how to avoid contamination? Also I need to know how many time passage the primary cells will remain active and proliferate?
You can find all answers to your questions in this source “Protocols for the growth and maintenance of CEF cells in the laboratory. Curr. Protoc. Microbiol. 17:A.4I.1‐A.4I.8. © 2010 by John Wiley & Sons, Inc.”
You can find all answers to your questions in this source “Protocols for the growth and maintenance of CEF cells in the laboratory. Curr. Protoc. Microbiol. 17:A.4I.1‐A.4I.8. © 2010 by John Wiley & Sons, Inc.”
sorry i tried to log in to wiley library but unfortunately i could not be able to access so please who has this paper send it to me : “Protocols for the growth and maintenance of CEF cells in the laboratory. Curr. Protoc. Microbiol. 17:A.4I.1‐A.4I.8. © 2010 by John Wiley & Sons, Inc.”
Mohamed,
Sending this publication to you by anybody for RG (with exception of the authors of this work) would be in violation of the copyright rules. If your library does not have this book, then I would suggest you may ask your librarian for an interlibrary loan. Alternatively, you may try requesting the reprint directly from the authors. Good luck!
I am sorry but i did not mean this, but i asked if any one could log in to the library and send this for me. this will be a kind of him but i did not mean to be illegal, but i am sorry for my misunderstanding
Hi,
please see following link:
www.youtube.com/watch?v=T_IsjJVv0PM
Good luck
thank you Dr Mansoureh i watched this video, but i need another video for the steps after that video.
THANK YOU VERY MUCH FOR YOUR SUPPORT I GOT THE CEF MONOLAYER AT LAST
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