Mohamed S Ahmed

9 Questions 39 Answers 0 Followers

Questions related from Mohamed S Ahmed

How do I prepare a primer of 100 µM stock solution?

Today i received a GAPDH- AB, MdCv-F and SNV-LTR-F/R primers forward and reverse and this is the first time for me to make a PCR reaction and I think I need to prepare a 100 µM stock solution...

02 February 2015 1,340 8 View

Do I need the peroxidase blocking step in the case of immunocytochemistry?

I have some tissue cultures cells and I want to perform IHC for these cells using DAB as a chromogenic solution. I want to ask if the peroxidase blocking is necessary in this staining or not?

09 September 2014 3,609 10 View

How to prepare primary chicken embryo fibroblast?

I need to know how to prepare primary chicken embryo fibroblast and how to avoid contamination? Also I need to know how many time passage the primary cells will remain active and proliferate?

06 June 2014 2,637 11 View

What is the procedures to infect a healthy cell culture with another viral infected cell culture?

I have 5 dishes of Chicken embryo fibroblast (CEF) culture infected with virus and I want to infect another new CEF by the previously infected cell culture. Could someone tell me how to do this...

07 July 2013 4,744 2 View

What could cause the detachment of Chicken embryo fibroblast (CEF) inoculated with MD virus?

I inoculated MD virus into CEF but the I found the cell sheet was detached in one of my dishes

07 July 2013 9,827 3 View

how can I recognize the hayflick limit for a certain tissue culture under Microscope

nowadays I am working on chicken kidney cell culture and CEF and i want to know how to know that these cells reach the hayflick limit and after this what i can do for these cells to make such...

07 July 2013 1,653 8 View

How I can get rid of the fungal contamination?

I have fungal contamination in my incubator and I want anything that may help me to get rid of this contamination because it's bothering me in cell culture or any work inside incubator. I washed...

07 July 2013 8,351 17 View

I want to know how to construct a new tissue culture or cell line from a certain tissue.

I want to construct a new cell line from tumorous cells.

02 February 2013 7,457 9 View

Is there any available cell line or tissue culturing for Psittacine beak and feather disease?

This is a viral disease belonging to family Circoviridea and it considered the smallest viral particle among the avian pathogens. Until now it had been difficult to find a cell line for its...

02 February 2013 4,814 2 View