No title

01 February 2015 1,284 8 View

Do I need the peroxidase blocking step in the case of immunocytochemistry?

I have some tissue cultures cells and I want to perform IHC for these cells using DAB as a chromogenic solution. I want to ask if the peroxidase blocking is necessary in this staining or not?

08 September 2014 3,541 10 View

How to prepare primary chicken embryo fibroblast?

I need to know how to prepare primary chicken embryo fibroblast and how to avoid contamination? Also I need to know how many time passage the primary cells will remain active and proliferate?

05 June 2014 2,582 11 View

What could cause the detachment of Chicken embryo fibroblast (CEF) inoculated with MD virus?

I inoculated MD virus into CEF but the I found the cell sheet was detached in one of my dishes

06 July 2013 9,773 3 View

What is the procedures to infect a healthy cell culture with another viral infected cell culture?

I have 5 dishes of Chicken embryo fibroblast (CEF) culture infected with virus and I want to infect another new CEF by the previously infected cell culture. Could someone tell me how to do this...

06 July 2013 4,692 2 View

how can I recognize the hayflick limit for a certain tissue culture under Microscope

nowadays I am working on chicken kidney cell culture and CEF and i want to know how to know that these cells reach the hayflick limit and after this what i can do for these cells to make such...

06 July 2013 1,600 8 View

How I can get rid of the fungal contamination?

I have fungal contamination in my incubator and I want anything that may help me to get rid of this contamination because it's bothering me in cell culture or any work inside incubator. I washed...

06 July 2013 8,283 17 View

I want to know how to construct a new tissue culture or cell line from a certain tissue.

I want to construct a new cell line from tumorous cells.

01 February 2013 7,407 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View