Mansoureh Hashemi

3 Questions 9 Answers 0 Followers

Questions related from Mansoureh Hashemi

What is the suitable medium for transfecting HEK 293 cells with calcium phosphate?

I am using calcium phosphate for transfecting Hek 293T cells to produce lentivirus-GFP. It is best that transfection medium be DMEM or DMEM/F12. Does this medium have HEPES or not? Does HEPES...

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What is best solvent of MTT powder for cell viability assay?

I want to assay cell viability with MTT powder from Merck company. What is best solvent of MTT powder for cell viability assay? Thanks.

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How can I avoid fibroblast growth in the culture of human brain tumors?

I am working with human brain tumor (grade IV: glioblastoma multiforme). The brain tumor has 70% glioma and 30% fibroblast cells in the culture. How can I avoid fibroblast growth and obtain a...

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