problem with how much quantity should be add of 50 mM NaCl in 750 mM Tris Buffer
Hi there,
The easiest is to first prepare stock solution for each component: we usually do 1M Tris-HCl pH7.4 and 5M NaCl. From those, mixing 3/4 of the former and 1/100 of the latter and adjust the volume with water will give you the buffer. BTW, 750mM is a very concentrated Tris buffer...
Dear Ghanshyam
1. Dissolve 90.85 g Tris base and 2.92 g NaCl in deionized water (slightly less than 1000 ml).
2. Adjust pH to 7.4 with HCl 1N and NaOH 1M. Bring final volume to 1 Liter with deionized water.
3. Autoclave and store at room temperature (RT).
Best, Roshdi
Hi everyone.
Thank you Katja Härtl and Mehdi Roshdi Maleki , it also very useful for me.
So, Dear Ghanshyam Jangir After calculate the different necessary masse, of each reagent as Katja Härtl said just mix them as Mehdi Roshdi Maleki said.
BTW, can I use the same way to prepare 50 mM Tris (8.0), 10 mM EDTA, 100 µg/mL RNAse ?
Thank you.
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