I am first time working with Pythium sp. I got few media from research articles such as V8 agar, Corn meal agar, NARF, NARM etc. I want to know which one is better to use for the growth of Pythium ultimum?
Dear Monika,
For isolation: PARP medium has been the most commonly used.
For growing: CMA is the best with Ampicillin.
Carrot agar and vegetable oil nitrate agar.
I recommended you check this book, plant pathological methods
Fungi and Bacteria.
Dear, Monika Jangir,
You can use the media CMA, MEA and OMA, they are good media for growing Pythium ultimum.
Also, you can use PDA medium at a temperature degree of 25 C, but without adding the antibiotic Chloramphenicol, because it prevents the fungus growth.
If you want to isolate the fungus Pythium from the infected plant roots, you can prepare a water culture by putting pieces of roots in these plates and incubating them for 2-3 days. After that, when you see the mycelium, you must transfer it to plates containing sterile PDA medium (without antibiotic) and incubating the plates at 25 C for 3-4 days in incubator.
V-8 Agar medium is also used successfully for isolating Pythium. This medium consists of 8 types of vegetable including carrot, spinach, tomato, celery, parsley, beets, watercress and lettuce. They are mixing together with CaCO3 with known percent and preparing a juice from them.
If you want to know the contents of V-8 Agar medium, I list it's contents as below:
You can also add 1 gm Sucrose to the above content to get a good growth.
Hope you can prepare it successfully.
With best regards.
PARP medium has been the most commonly used for isolation of Pythium spp.
Several media, such as OMA, CMA, PARP, MEA, PDA have been employed to isolate Pythium spp.
Have you ever tried Sabouraud dextrose agar, Brain heart Infusion agar and Pal sunflower seed medium for the isolation of Phythium spp.?
We have published one paper on Pythiusm insidiosum that you can easily download from the Research Gate.
You can also read Pythiosis in my book entitled "Veterinary and Medical Mycology".
Dear, Kulvinder Bajwa,
What do you mean by the medium PARP?
And how to use it please?
With best regards.
Hi Dr Monika Jangir . I hope the following link could help:
https://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1985Articles/PlantDisease69n05_393.PDF
Do you mean the medium which consist of (Pimarcin Ampicillin Rifampicin Pentachloronitrobenzens)?
Yes, it can be used for isolation of Pythium and Phytophthora successfully.
It consists of the following compounds:
Pimaricin 5 mg Ampicillin 250 mg Rifampicin 10 mg PCNB 100 mg
You can check the below reference:
(Jeffers, S. N. and Martin, S. B. (1986). Comparison of two media selective for Phytophthora and Pythium species. Plant Disease, 70:1038-1043).
You can also use NARF (Nystatin, Ampicillin, Rifampicin and Fluazinam)
Nystatin 25 mg Ampicillin 150 mg Rifampicin 5 mg Omega 500 5 µl (Syngenta; 40% fluazinam)
Please see the reference below:
Morita, Y. and Tojo, M. (2007). Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil. Plant Disease, 91 (12):1591-1599.
Check the attached link:
https://plantpath.psu.edu/pythium/module-2/media-for-pythium-culture
Yehya A. Salih sir, why we have to use sterile PDA medium (without antibiotic) while culturing Pythium.
Thank You.
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