If a gene have multiply locations on a chromosome, does it then matter which primer sets I choose? And if yes, how do I find which location I want to use for my primers? The gene is connective tissue growth factor (CTGF) from a pig. I want to detect it in the pigs stomach.
Oscar L. Sierra
if the coding sequence is identical and there's no alternative splicing mechanisms, no, it doesn't matter.
If there are neutral changes in the coding sequence (SNPs), these might affect how the primers partially anneal
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