I will detect 3 different genes in qPCR. Is it necessary to have a RT and NCT control for each gene? The only different in the samples is only the primers to qPCR, so I don't think the control for each gene seems so important. ?
Mohamed-Mourad Lafifi thank you, but I have read the most of these attachments before, and don't think they describe weather I should have a RT-control for each of my 3 genes. - they just says I should have one.