12 Questions 11 Answers 0 Followers
Questions related from Amalie Storm
Hi, I want to analyze my qPCR (gene expression, mechanical tested and not testet) results, and have to normalize them to my reference gene GAPDH. I don't have a standard curve. I have looked at...
27 April 2021 4,750 4 View
I will detect 3 different genes in qPCR. Is it necessary to have a RT and NCT control for each gene? The only different in the samples is only the primers to qPCR, so I don't think the control for...
19 April 2021 5,306 3 View
I have taken over a qPCR protocol from a former student. In this one, she uses purified RNA as a control, but I can not figure out why or whether it is a positive or negative control. I do gene...
13 April 2021 1,055 10 View
If a gene have multiply locations on a chromosome, does it then matter which primer sets I choose? And if yes, how do I find which location I want to use for my primers? The gene is connective...
26 March 2021 7,191 1 View
Hi, I want to analyze the CTGF (CCN2) gene in qPCR from stomach tissue from a pig. I have found some primers here:...
24 March 2021 5,327 0 View
23 March 2021 5,474 1 View
I have to run qPCR on RNA from mucosa from a pig's stomach. I have now tried 2 times without success. My RNA concentrations are too low to make cDNA. I get my stomach from a butcher 40 minutes...
11 March 2021 6,871 5 View
01 January 1970 5,683 5 View
I was told my low RNA-concentrations extracted from a pigs stomach could be due to the amount of RNases in the stomach. I was not cut up at the slaughterhouse, so it contained all the stomach...
01 January 1970 6,131 1 View
I was told my low RNA-concentrations extracted from a pigs stomach could be due to the amount of RNases in the stomach. I cant find it in the litterature or on google. Can someone help? I need a...
01 January 1970 4,915 1 View
01 January 1970 6,979 1 View
01 January 1970 6,245 0 View
