Ok, you just spin with 13,000 rpm for 5 seconds then other organic compounds will go down to the bottom of tube but DNA still contain with the supernatant. By the way, very important thing is, make sure that the material you collect (leaves of a tree) is not too much (just a small piece it is enough for performing PCR).

Yes, I already spinned down all big pieces before continuing extraction with lysis buffer and after lysis buffer, but even after these steps the sup was green. About the amount I need to obtain some milligrams of DNA but not for pcr....

hi! try with this protocol is too easy and the DNA is very clean

A simple and efficient method for isolation of DNA in high mucilaginous plant tissues

Molecular Biotechnology

October 2005, Volume 31, Issue 2, pp 129-135

I'd like to try it: do you have the pdf? Anyway for the moment I compared salting out and ctab and the ctab is cleaner (I make two chloroform extraction instead of one).even if I still observe a yellow pellet...

Hi! This is the pfd..

It is important that the amount of tissue you are extracting from is not too much. If so, your sample will be very contaminated with lipids and proteins. That is why you are getting a yellow pellet. For CTAB, only a small piece of a leaf is necessary. If you need a high amount of DNA, you can always use PCR to amplify what you extract.

thank you naivy for the pdf and thank you lauren for suggestion. yes I also was thinking that the problem is the amount. however I need the entire genome then I cannot resolve with a PCR. I am trying to dilute more the initial sample (two-three Leaves grounded) with more CTAB solution and to make twice the chloroform extraction... it seems that the quality improved