Kelly Avila suggested a good method. However, the composition of CTAB buffer can be modified by adding 1%PVP (Polyvinylpyrrolidone) which minimizes the interference of secondary metabolites during DNA extraction from plant leaves.

Kelly Avila suggested a good method. However, the composition of CTAB buffer can be modified by adding 1%PVP (Polyvinylpyrrolidone) which minimizes the interference of secondary metabolites during DNA extraction from plant leaves.

thank you all. so it seems that ctab is the gold standard for plant DNA extraction. do you know also if there is any good kit? my priorities are both high purity and amount....

Qiagen has a pretty good kit, its called DNeasy Maxi kit. I have used mini kit, and the results were as promised in their instruction book (which is pretty rare to be honest). CTAB is the gold standard, no question about it, but kits make the process pretty fast.

Can you please tell me from which plant leaves do you want to isolate DNA?

And, what is your downstream application?

Hi, I used a kit from Epicentre. It's much cheaper than Qiagen and I had similar amount of DNA as with the extraction kit from Qiagen.

Have a look here: http://www.epibio.com/applications/nucleic-acid-purification-extraction-kits/dna-extraction/quickextract-plant-dna-extraction-solution

Cheers, Nadine

thank you. I could try to compare the kit suggested by nadine with doyle&doyle protocol to see if there is a big difference in terms of amount\quality

Hello, I tried the Doyle and Doyle protocol and a salting out protocol for DNA extraction. The supernatant is in both cases dark green (I extracted from mature leaves of a tree). Does anyone know how to overcome this problem in irder to get a clearer sup?

Try to use young leaves for your extraction process. If your sample is known to contain high amounts of secondary metabolites then you might have to use a modification of the Doyle and Doyle protocol.

Regarding green supernatant, try to extract twice with chloroform (might help). But, if u have funds....buy a kit.