I have extracted DNA (proteinase k, chloroform extraction followed by isopropanol and ethanol 70) from a biological fluid and checked its purity with nanodrop: unfortunately the values of these ratios were very low: the spectrum presents two peaks at 230 and 280 with a valley at 260. Apart from searching for another protocol for extraction, in the meanwhile could I try to purify these DNAs? Thanks
I'm affraid valleys at 260 nm are not promising whichever the ratio is, and it's going to be below 1.
@nadine ratios are below 1...when I extract I can observe a pellet and on the agarose gel there is a faint smear around 1.5 and 2.5 kb... @ted I tried to use proteinase K before extraction but the result was exactly the same. I cannot use silica columns, because when I tried to extract with a kit I did not get anything...
the abs at both 260 and 280 are high, but a230 and a280 are pretty much higher in comparison with a260: that's why I see a valley at 260; in addition, when I read the raw fluid at nanodrop I get the same results in terms of absorbances. However, on the gel I can detect a faint smear of few kb.
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