Are there experiments with co-culture of tumor and normal cells?

does someone know if it exists an experiment where tumoral and non tumoral cells have been cultured together? if yes, please link me the literature! thank you

03 April 2015 9,984 11 View

Is it possible to purify nucleic acids from a solution containing vitamins and other compounds with UV absorbance spectrum?

I have tried to extract nucleic acids from a solution containing such compounds, and as I said in another question, I got very high A230 and A280 values. I was wondering if I can get rid of these...

04 May 2014 4,932 1 View

Methods for changing the buffer of a DNA solution?

I have some low molecular weight DNA in a highly saline buffer and I would like to resuspend it in water or TE. The only methods that I know are precipitation and dialysis but I would like to...

03 April 2014 6,110 6 View

Absence of contaminant nucleic acids when extracting DNA from plant leaves - any thoughts?

I am extracting DNA from leaves taken from a tree growing in the field. I was wondering how I can be sure that DNA extracted comes only from the plant and not from contaminating agents (mainly...

03 April 2014 8,806 4 View

Can anyone offer advise regarding low A260/A230 and A260/A280 ratios?

I have extracted DNA (proteinase k, chloroform extraction followed by isopropanol and ethanol 70) from a biological fluid and checked its purity with nanodrop: unfortunately the values of these...

03 April 2014 9,870 4 View

How to obtain a very high amount of plant DNA?

Does anyone have an idea of how much plant tissue (Leaves grounded in liquid nitrogen) should be processed in order to get at least 1 gram of DNA by classical extraction methods (CTAB)?

03 April 2014 7,668 11 View

How to obtain a clear supernatant from plant leaves DNA extraction?

I tried the CTAB protocol and a salting out protocol for DNA extraction from mature leaves of a tree. The supernatant is in both cases dark green and I suppose that DNA solution still contains...

02 March 2014 1,785 7 View

Could anybody suggest a smart method/kit /publication with a tested protocol to get milligrams of DNA high quality from plant leaves?

Thank you very much for answers.

31 December 2013 2,857 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View