I have tried to extract nucleic acids from a solution containing such compounds, and as I said in another question, I got very high A230 and A280 values. I was wondering if I can get rid of these vitamins so that DNA can successively be sequenced. I tried with classical extraction and a couple of kits.
Dear Artur, usually I thank people for their answer and give answers to the questions that are asked to me (apart if I forget). I am not going to downvote you, and be sure i'm not hurt at all.
Yes,the first thing to do is to discuss with colleagues, who tells you that I did not? If I have decided to write here maybe we could not have found a solution yet. On the other side, the aim of this network is to exchange ideas and experiences among researchers of different fields that you could not have in an institute.
Coming back to my question, when I extracted this DNA, found mountains at A230 and A280 but I could see a very faint DNA band in ethidium bromide stained gel. This was confirmed to me by two colleagues working independently.
I am almost sure that vitamins, given that they are present in this solution and given their absorbance in the UV (as I wrote here), could be responsible at least of the peak at 230, whereas in last question I did not know it yet.
About the result with kits (one from promega and the other from qiagen for DNA gel extraction) I thought it was clear from this question that were always the same, with dna yield very reduced, in addition.
We decided not to make PCR, but direct sequencing, however we do not know if these impurities will give troubles, that's why I also asked if someone has experience about that.
do you need further explanations? thank you anyway for your answer and...suggestions.
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