Annalisa Varriale

9 Questions 29 Answers 0 Followers

Questions related from Annalisa Varriale

How to retrieve sequence coordinates and other info from geo data?

Hello, could someone suggest me a tool to get information (gene localization, DNA methylation, CG composition and other ) from geo data? Thanks

11 November 2017 1,037 4 View

Are there experiments with co-culture of tumor and normal cells?

does someone know if it exists an experiment where tumoral and non tumoral cells have been cultured together? if yes, please link me the literature! thank you

04 April 2015 10,027 11 View

Is it possible to purify nucleic acids from a solution containing vitamins and other compounds with UV absorbance spectrum?

I have tried to extract nucleic acids from a solution containing such compounds, and as I said in another question, I got very high A230 and A280 values. I was wondering if I can get rid of these...

05 May 2014 4,974 1 View

Methods for changing the buffer of a DNA solution?

I have some low molecular weight DNA in a highly saline buffer and I would like to resuspend it in water or TE. The only methods that I know are precipitation and dialysis but I would like to...

04 April 2014 6,155 6 View

How to obtain a very high amount of plant DNA?

Does anyone have an idea of how much plant tissue (Leaves grounded in liquid nitrogen) should be processed in order to get at least 1 gram of DNA by classical extraction methods (CTAB)?

04 April 2014 7,714 11 View

Absence of contaminant nucleic acids when extracting DNA from plant leaves - any thoughts?

I am extracting DNA from leaves taken from a tree growing in the field. I was wondering how I can be sure that DNA extracted comes only from the plant and not from contaminating agents (mainly...

04 April 2014 8,853 4 View

Can anyone offer advise regarding low A260/A230 and A260/A280 ratios?

I have extracted DNA (proteinase k, chloroform extraction followed by isopropanol and ethanol 70) from a biological fluid and checked its purity with nanodrop: unfortunately the values of these...

04 April 2014 9,921 4 View

How to obtain a clear supernatant from plant leaves DNA extraction?

I tried the CTAB protocol and a salting out protocol for DNA extraction from mature leaves of a tree. The supernatant is in both cases dark green and I suppose that DNA solution still contains...

03 March 2014 1,832 7 View

Could anybody suggest a smart method/kit /publication with a tested protocol to get milligrams of DNA high quality from plant leaves?

Thank you very much for answers.

01 January 2014 2,908 9 View