Hi all, I have SEM images of electrospun nanofibers and I'd like to measure its porosity/diameter. However I'm not sure how to do it with ImageJ or any other software. Can anybody help me please? Thanks in advance
Method 1.. . Please check the manual on ImageJ home-site.
https://imagej.nih.gov/ij/docs/guide/146-30.html#sub:Measure...[m]
It can be done with image j. To do it basically :
It is also possible (if you have good contrasts, etc), to get ImageJ to measure many pores automatically and give a histogram.
This depends on 3 things 1) Resolution of your microscope 2) Magnitude of your pores 3) Clear images where you can see all the porous structures one by one
Or
Method 2. you can install the "DiameterJ" plugin. It calculates pores, fiber diameter and orientation.
If you spin PLA in CHCl3 you might have fibers big enough for nanoCT scanners. Then you have the real scaffold pore sizes rather then 2D ones.
Or
Method 3. For porosity measurement you can go for liquid displacement method or BET rather than image analysis.
Regard Zia Ullah khan
Hi Shaini, There are some excellent videos on youtube on this topic. Have a look you will become a pro in it, in less than an hour.
There is a video reference where comparison of nanofiber diameter with ImageJ and SEM software have been briefly explained.
Source: https://youtu.be/TmQwKs5GmbU
Hello dear, you can measure porosity by uploading image in Autocad program and then specifying the distance between the boundaries of each porous by using roller tool then the number will be shown. Finally to get exact unit numbers you must convert image scale to the same scale in SEM image, then compare exactly with shown number of porosity diameter.
These could help:
https://www.youtube.com/watch?v=e2j5dZ5iJ_8&list=RDe2j5dZ5iJ_8&start_radio=1&t=33&t=33
Thank you all for your suggestions. I shall give them a try and see how it goes
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