Shalini Paul

10 Questions 6 Answers 0 Followers

Questions related from Shalini Paul

Exosomal protein extraction protocol?

Hi all, I've been isolating exosomes from cell culture media using ultrafiltration (protocol recommended by Merck). So far, I'm getting good number of exosomes. However, I'm having a bit of...

05 January 2021 9,816 6 View

How to measure porosity/diameter of electrospun nanofibers using imagej?

Hi all, I have SEM images of electrospun nanofibers and I'd like to measure its porosity/diameter. However I'm not sure how to do it with ImageJ or any other software. Can anybody help me please?...

28 July 2020 6,416 7 View

Significance of pAkt/Akt and pErk/Erk ratio?

Hi, I'm testing the effect of a drug on cardiomyocytes. The treated cells are then used for Akt and Erk ELISA. Can anyone tell me how do I interpret the pAkt/Akt or pErk/Erk ratio? For...

11 September 2018 4,136 3 View

Exosome markers for HUVECs?

Hi, I have been isolating exosomes from HUVECs culture and wanted to run a western blot for the characterization of exosomes. Till date, I have only checked for CD63 marker and it has worked....

01 January 1970 6,380 3 View

Significance of Calnexin in exosome samples?

Hi all, I was doing a western blot with my exosome samples, and I found the presence of Calnexin in them. Now, I know technically it is not supposed to be in there, since Calnexin is a negative...

01 January 1970 7,253 3 View

Exosome isolation using Amicon ultracentrifugation filters?

Hi all, I'm trying to isolate exosomes using Amicon UFC filters. To do that, I'm using media collected from HUVECs. Has anybody tried this type of isolation or know how it works? Please let me know.

01 January 1970 6,540 2 View

Trizol removal from cell samples and resuspension in lysis buffer??

Hi, I have received a few samples in Trizol at -80C, and I want to do a protein extraction for it. Now, I know that extraction using Trizol does not give good results, so is it possible to remove...

01 January 1970 8,350 2 View

Significance of CD63 markers in exosomes?

Hi, I was running Western blot with exosome lysates (exosomes obtained from HUVECs) and probed them with CD63 primary antibodies. However, sometimes I'm getting bands around 27 kDa, while other...

01 January 1970 8,180 0 View

Calnexin bands detected between 37-50kDa?

Hi, I was running my Western blot with cell lysates and exosome samples and exposed the blot to Calnexin primary antibody (1:500 dilution). As you may know, Calnexin (67 kDa molecular weight) is...

01 January 1970 8,981 0 View

Protein extraction protocol from cell-derived exosomes?

Hi, I'm culturing primary HUVECs for exosomes. When the cells reach 70-80% confluence, I serum starve them for 24 hrs, then collect cells and media. Now from media, I isolate the exosomes first,...

01 January 1970 2,788 1 View