The reviewer requested that the authors indicate the detection limit for the viral titers assay. However, the assay was performed using PFU, and I am unsure how to determine the assay's detection limit.
Can someone understand the reviewer's request?
Coul help me? Please?
Dear Carolina,
The detection limit may refer to the minimum or the maximum number of viable phage you can detect using (e.g.) a soft-agar plating method, and both can be valuable for different reasons.
The maximum detection limit is simply the highest dilution of your suspension which produces one or more plaques. If you tested a 1/1,000 dilution (and saw one or more plaques) and a 1/10,000 dilution (and saw no plaques), the maximum detection level for an assay that only went up to a 1/10,000 dilution would be 9,999 PFU (or
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