What to use to disrupt meiotic spinde during IVM?

31 October 2022 0 2K Report

Hi everyone!

I would like to perform an experiment on meiotic metaphase I (MI) spindle in mouse oocytes and I am looking for a substance to add to the maturation media M2, able to impede/disrupt spindle formation for the whole duration of maturation to metaphase I. In the past I used Monastrol [10 µM], but in that case I treated the oocytes post-maturation for only 1h.

Do you have any reference of Monastrol used for the whole IVM maturation? What othe substance may I use to dsrupt the meiotic spindle?

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