What to use to disrupt meiotic spinde during IVM?

31 October 2022 0 2K Report

Hi everyone!

I would like to perform an experiment on meiotic metaphase I (MI) spindle in mouse oocytes and I am looking for a substance to add to the maturation media M2, able to impede/disrupt spindle formation for the whole duration of maturation to metaphase I. In the past I used Monastrol [10 µM], but in that case I treated the oocytes post-maturation for only 1h.

Do you have any reference of Monastrol used for the whole IVM maturation? What othe substance may I use to dsrupt the meiotic spindle?

Badges
Science topic

More Davide Bosi's questions See All

Incubation of antioxidants with viral inoculum experiment?

I would like to understand whether a specific frequency of light (in the visible spectrum) inactivates a viral species by oxidative process through ROS formation or by a conformational change of...

05 May 2024 2,109 0 View

Polymers pyrolysis using "PYROLYSIS REACTION"?

Hello everyone, I am trying to use the new type of reaction present in Aspen plus V14, known as "pyrolysis." I have tried to simulate pyrolysis of HDPE, by entering the kinetics suggested by...

03 April 2024 2,086 0 View

Find a controller for a feedback-loop?

My team and I are working on an automation project to stabilize the altitude of an airplane. However, we've encountered an issue that seems unsolvable for us, at least for the moment, in the final...

03 March 2024 7,659 0 View

How to transfer data between host PC and Win95 in a Virtual Box?

I have installed Windows 95 in a Virtual Box on a Windows 11 PC. How can I import and export data in Win 95?

13 February 2024 5,427 2 View

How do I induce heavy metals stress in potted plants?

I am currently researching the stress physiology of photosynthesis. I have previously worked with hydroponic systems using heavy metals at concentrations around tens of microMolars. Now, I would...

02 November 2023 4,163 1 View

How does accurate classification and diagnostis of different types of anemia using laboratory tests impact patient management and treatment strategy?

Medical Technology question

06 September 2023 1,851 5 View

APARC + ASEG: where to find the nifti file and nomenclature of the ROIs?

Dear neuroimaging experts, I am trying to find the APARC+ASEG nifti file used by freesurfer, along with a file / table with the nomenclature of the ROIs (i.e., list of ROIs with labels). Could...

29 May 2023 9,239 1 View

Help with triple immunofluorescent staining?

I am currently optimising a protocol for triple staining of murine wounds. I am using MAC-3 (rat), Arg-1 (mouse) and iNOS (rabbit) to characterise the macrophage population in the wound. As...

16 January 2022 3,819 0 View

Metanephrines in adrenal incidentaloma turnaround time?

Dear all, in guidelines about adrenal incidentalomas ( doi.org/10.1530/EJE-16-0467

12 January 2022 4,215 0 View

Where I can find Ansys Solid185 theoretical formulation?

I am looking for a theoretical formulation of solid185. In particular, I want to understand which equations are implemented to build the stiffness matrix.

04 July 2021 8,558 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View