particularly in relation to the oocytes evaluation criteria and the used medium, I mean a recent used for IVM, IVF and IVC for culturing of bovine embryo to the blastocyst stage.
Dear Haitham, please see our attached paper regarding using 2 step defined medium for IVC. Our recent results showed 40-45% of BL production.
Many thanks Islam, I already got this paper, however, the high blastocyst rate that I meant in which excedding 60% .
Thanks again
https://www.jstage.jst.go.jp/article/jvms/64/11/64_11_967/_pdf
VEGF + mSof medium.
http://www.scielo.br/scielo.php?pid=S1516-35982014000300003&script=sci_arttext
total cell numbers (control - 109.53 vs. treated - 120.67 for bovine
Melatonin Improves the Quality of In Vitro Produced (IVP) Bovine Embryos: Implications for Blastocyst Development, Cryotolerance, and Modifications of Relevant Gene Expression. Melatonin and bovine blastocyst
Take a look in this one:
Establishment of An Advanced Chemically Defined Medium for Early Embryos Derived from In Vitro Matured and Fertilized Bovine Oocytes.
https://www.jstage.jst.go.jp/article/jrd/57/6/57_11-039H/_pdf
Many thanks Michel and Eliza for your response and the articles. I`m sure that sharing scientists the newly developed medium and culture system will lead to the great progression of in vitro production aspects.
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