Digital

Review and cite DIGITAL protocol, troubleshooting and other methodology information | Contact experts in DIGITAL to get answers

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Questions related to Digital

Do the extracted RNA from human whole blood using QIA amp RNA blood mini kit, need to clean up ?

last month 5,220 3 View

What is the recommended protocol or composition to prepare an effective RNase Kill solution in the lab for RNA work? Are there any reliable recipes ?

I want to know how to make an effective RNase Kill solution in the lab to prevent RNA degradation during experiments.

last month 5,744 2 View

What role can ICT-based platforms play in enhancing coordination and knowledge sharing among research institutions, extension and farmers in UP ?

What role can ICT-based platforms play in enhancing coordination and knowledge sharing among research institutions, extension agencies, and farmers in Uttar Pradesh?

last month 5,414 3 View

How can explainable AI (XAI) improve trust, fairness, and regulatory compliance in credit scoring models for thin-file consumers?

In the growing adoption of AI-driven credit scoring models, particularly for thin-file consumers—individuals with limited or no traditional credit histories—explainability and transparency become...

27 June 2025 338 4 View

Is reprogramming PBMCs of older individuals into iPSCs more difficult? Does anyone have experience in this?

Hi, I have successfully reprogrammed PBMCs of 20-30 year old individuals into iPSCs before, using the Sendai virus based Cytotune 2.0 kit. However, I am not getting any colonies from PBMCs of...

17 June 2025 959 3 View

Teachers would need information if there were clear methodologies on the main selection methods in fruit growing and the procedure for their ?

I want to create a variety in the fruit industry, it must be done precisely.

07 May 2025 5,802 3 View

Why I cannot upload two Lancet papers I have co-authored?

Dear Sir/Madam, I cannot upload two Lancet papers that I have co-authored. Both papers have more than 700 and 500 citations, so they are really important to me. I am providing below the title...

03 May 2025 4,922 2 View

Recommend an optimized protocol for improving protein yield at 37 °C?

I have successfully transformed E. coli BL21 (DE3) cells with a plasmid encoding my desired mammalian protein. The transformed cells were cultured in LB medium at 37 °C, and protein expression was...

19 April 2025 1,069 3 View

Should I conduct an RRL before qualitative interviews?

Hello. The panel advised me to use a sequential exploratory design for scale development. However, the construct that I will be studying is from the 70s. Also, there are existing scales about it....

19 March 2025 221 3 View

I treid to create ipsc from PBMC using sendai virus , I tried twice , but I didnt get the colonies , This is my second , Can some one help ?

I tried twice to create ipsc. first time I saw cells, but I was not sure whether they were colonies,I attached the images of Ist and second trial , I hope someone can clear my doubts

10 March 2025 7,480 2 View

Can we process 1000 observations using Smart PLS?

I need to process more than 1000 observations. Can I do it using smart PLS ? Expert opinion pls

02 March 2025 4,506 2 View

When and how do I know if I have been granted access to a PDF?

I can't seem to find the page that answers that question

18 February 2025 2,952 1 View

Small-signal stability analysis?

IS THERE ANY BENCHMARKING POWER SYSTEM SMALL-SIGNAL STABILITY SIMULATION TOOLS? (between SSAT and DigSILENT-PF)

13 February 2025 9,963 0 View

Do you know of any free journal in education , technological integration, SDGs, Language education, Artificial intelligence, digital literacy?

Do you know of any free Scopus-indexed/ DHET or DOAJ journal or book in education , technological integration in education, SDGs, Language education, Artificial intelligence, digital literacy,......

06 February 2025 9,217 1 View

Is it possible for a value of Chi-square/df that is higher than 5 when testing CFA in AMOS?

I am evaluating a structural measurement through CFA with 7 variables. The model fit indices are good, except for the value of CMIN, which is 5.790 > 5, violating the criteria. Sample size =...

22 January 2025 8,253 3 View

Why Do I See Higher Methylation Percentages in Bismark Extraction Results?

I am new to bioinformatics and i am currently working on my whole-genome bisulfite sequencing (WGBS) data to analyze DNA methylation patterns. The data involves paired-end sequencing processed...

21 January 2025 6,079 1 View

How to deal multimodal data with longitudinal design?

If I want to analyze multimodal data collected over multiple time periods and form results similar to network mechanisms, how should I proceed, and what data processing and statistical methods...

18 January 2025 5,274 1 View

Why do my MEF cells have these black dots in them?

I tried working with MEF cells and first few seedings were fine. I started seeing these tiny black dots under microscope after 24 hours. I also kept media separately for checking any kind of...

10 January 2025 5,123 0 View

What is the difference between theory development, testing, and confirmation in accordance with Partial Least Squares (PLS)?

When introducing new constructs or variables into a model and examining their effects in accordance with specific established theory, especially in a context where the theory has not been tested...

24 November 2024 4,963 5 View

After performing multiple sequence alignment, how do i analyze the conserved motifs and domains?

Multiple sequence alignment of five species of HSP 70 protein was done by Clustal omega. I did performed conserved domains from CDD of NCBI. How to analyze the conserved motifs and domains and...

21 October 2024 2,704 2 View

Can ozone nanobubbles independently produce hydroperoxide radical (HO₂⋅)?

Is hydroperoxide radical (HO₂⋅) one of the many reactive oxygen species (ROS) produced by ozone nanobubbles?

18 October 2024 8,409 1 View

Porwer System recent Study??

power system recent study

09 October 2024 2,999 2 View

How much power Roger 5880 with thickness 1.6 mm and copper coating of 0.035mm can handle ?

What is the power handling capacity of Roger 5880

01 October 2024 3,930 0 View

How can we accurately induce polycystic ovary syndrome in rats?

Previous research suggests that some treatments, such as breast cancer treatments like letrozole, can cause an imbalance in sex hormone levels, leading to a condition similar to polycystic ovary...

29 September 2024 5,258 0 View